Höfundar:
Óttar Rolfsson, Hilmar Kjartansson, Aristotelis Kotronoulas, Adrian de Lomana, Christian Christiansen
Introduction
Acute thermal injuries requiring medical care are a leading cause of morbidity worldwide with an estimated 180,000 deaths every year. How biomaterials modulate the biochemical profiles of burn wound healing is not understood but of importance for understanding their mechanism of action and advance wound care. We are working towards understanding how fish skin grafts (FSG) influence lipid, metabolite and protein profiles of burn wound healing using a mass spectrometry based data driven approach.
Methods
Second and third degree burn wounds (n=4 for each type) were created on Yorkishire pigs (n=4) and treated with FSG or standard of care. Punch biopsies were collected during healing to allow monitoring of lipid mediators of inflammation, metabolites and proteins by untargeted UPLC/Q-TOF-MS.
Results
Lipid mediators derived from omega-3 fatty acids were increased at day 7 in the FSG treated wounds. However, prostaglandin F2a and its 15-keto derivative from the arachidonic acid pathway, along with 13-HODE and 13-HOTrE from the linoleic pathway, increased at day 7 independent of treatment. Differences in selected amino acids and nucleosides were also observed at day 7 in the FSG treated wounds. Changes in proteomics profiles during healing were characterized by the downregulation of proteins involved in keratinization and the immune response irrespective of wound severity.
Conclusions
Our results demonstrate that burn wound treatment with FSG can lead to the earlier formation of lipid mediators involved in the resolution of inflammation that is in part driven by changes to the protein and/or cellular composition of the wound.