Líf - og heilbrigðisvísindaráðstefna Háskóla Íslands 2021

New function of ATG7

Main author: Kevin Ostacolo
Company or Institution: University of Iceland

Introduction: Autophagy is a catabolic process evolutionarily conserved in eukaryotic cells. The process ensures a level of quality control within the cell thereby maintaining cellular homeostasis. A double membrane forms in the cytoplasm engulfing cargo for degradation, eventually resulting in the formation of an autophagosome. The autophagosome then fuses with the lysosome resulting in the degradation of the cargo within.

Methods: We previously identified an isoform of the essential autophagy gene ATG7, which cannot perform its characterized autophagy function. The shorter isoform of ATG7 lacks binding to proteins of the autophagy machinery whereas binding is gained with proteins of other cellular mechanisms.

In order to analyse the function of the shorter isoform, immunoprecipitation of ATG7 was performed followed by mass spectrometry analysis in HuH7 hepatocarcinoma cells and mouse embryonic fibroblasts.

Results and conclusions: This revealed different binding partners of the two isoforms of ATG7. The shorter isoform of ATG7 lacks binding to proteins of the autophagy machinery whereas binding is gained with proteins of other cellular mechanisms. Unravelling the isoform specific novel function of ATG7 is important for furthering our understanding of the autophagy process in health and disease.

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